Palestinian health officials and witnesses say Israeli forces fired on people as they headed toward an aid distribution site on Tuesday, killing at least 27, in the third such incident in three days.
The army said it fired "near a few individual suspects" who left the designated route, approached its forces and ignored warning shots.
The near-daily shootings have come after an Israeli and U.S.-backed foundation established aid distribution points inside Israeli military zones, a system it says is designed to circumvent Hamas.
The United Nations has rejected the new system, saying it doesn't address Gaza's mounting hunger crisis and allows Israel to use aid as a weapon.
The Israeli military said it was looking into reports of casualties on Tuesday.
It previously said it fired warning shots at suspects who approached its forces early Sunday and Monday, when health officials and witnesses said 34 people were killed.
The military denies opening fire on civilians or blocking them from reaching the aid sites.
The Gaza Humanitarian Foundation, which operates the sites, says there has been no violence in or around them.
On Tuesday, it acknowledged that the Israeli military was investigating whether civilians were wounded “after moving beyond the designated safe corridor and into a closed military zone,” in an area that was “well beyond our secure distribution site.”
The shootings all occurred at the Flag Roundabout, around a kilometer (1,000 yards) from one of the GHF’s distribution sites in the now mostly uninhabited southern city of Rafah.
The entire area is an Israeli military zone where journalists have no access outside of army-approved embeds.
"I start heading out at 3 a.m. and get there, then get shot at. So I lay low on the ground between the others. When it turned 5:03 a.m., I raised my head back up to see people walking out with aid boxes. But what about those martyrs left on the ground?," said Nahla Zareb, a displaced woman who went to collect aid.
An Associated Press reporter who arrived at the Red Cross field hospital at around 6 a.m. saw wounded people being transferred to other hospitals by ambulance.
Outside, people were passing by on their way back from the aid hub, mostly empty-handed, while empty flour bags stained with blood lay on the ground.
"I swear, it's like we died 500 times in an hour. I swear, there are martyrs left inside there that no one can reach, and I swear two thirds of the people who went inside (the distribution area) left empty-handed," said Ibrahim Saedi, a displaced man.
Israel ended the ceasefire in March after Hamas refused to change the agreement to release more hostages sooner. Israeli strikes have killed thousands of Palestinians since then, according to Gaza's Health Ministry.
Hamas-led militants killed some 1,200 people, mostly civilians, and took 251 people hostage in the Oct. 7, 2023, attack into Israel that ignited the war.
They are still holding 58 hostages, a third of them believed to be alive, after most of the rest were released in ceasefire agreements or other deals.
Israel's military campaign has killed over 54,000 Palestinians, mostly women and children, according to the Gaza Health Ministry, which does not say how many of the dead were civilians or combatants.
The ministry is led by medical professionals but reports to the Hamas-run government. Its toll is seen as generally reliable by U.N. agencies and independent experts, though Israel has challenged its numbers.
Israel says it has killed around 20,000 militants, without providing evidence. Around 860 Israeli soldiers have been killed since the Oct. 7 attack, including more than 400 during the fighting inside Gaza.
AP Production by Wafaa Shurafa