A reported single gunshot has sparked a fresh line of inquiry in the long-running manhunt for alleged police killer Desmond Freeman.

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Police conducted "firearms testing" near Barrett Lane and Rayner Track in Porepunkah, about 300km northeast of Melbourne in Victoria's northeast, on Wednesday as part of their search.

The testing was triggered by reports of a gunshot in the vicinity about 11.45 am on August 26.

Freeman had allegedly opened fire on police moments earlier, killing Detective Senior Constable Neal Thompson and Senior Constable Vadim

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