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Karen Read -- the Boston woman acquitted of killing her police officer boyfriend this year -- is suing several of the witnesses in the high-profile trial.

Read filed suit Monday against eight people who testified against her -- including the lead investigator, his two former supervisors, and five people who were present at the 2022 party that ended with John O'Keefe 's death.

Read was twice tried for murder in the death of O'Keefe -- her Boston cop boyfriend ... she was accused of striking him with her SUV on the night of a snowstorm, after a drunken party at the house where his body was later found in a snowbank in the front yard. She was ultimately acquitted of the murder and manslaughter charges ... but was found guilty of operating a vehicle under the influence.

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