Serial killers, baby murderers and arguably Australia's most infamous outlaw, bushranger Ned Kelly, were among the many criminals held and executed at Old Melbourne Gaol.

And some say the dimly lit, cold bluestone building, which operated as a prison between 1842 until its closure in 1929, is still "haunted".

That's especially the case for one particular room: cell 17 on the former jail's middle level.

"We get a lot of reports from people who have been pushed or shoved or even scratched and they show us scratch marks on their neck," said the National Trust's Kathleen Toohey, of the cell.

"Feelings of instant cold, cameras turn off and service dogs refuse to enter. We don't know why it could be haunted."

At least 133 people were hanged and buried at Victoria's oldest prison, including

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