YouTube TV viewers can no longer see Disney channels including ABC and ESPN after the two sides failed to agree on a new content distribution deal.
Other channels that vanished from Google's pay TV platform include the Disney Channel, FX and Nat Geo.
Google's pay TV platform said in a blog post late Thursday that Disney had followed through on a threat to suspend its content amid the negotiations.
The breakdown could impact coverage of some college football games on Saturday, as well as NBA, NFL and NHL games.
YouTube is the largest internet TV provider in the U.S. with more than nine million subscribers. Hulu, owned by Disney, is next, with about half that many.
Viewers have become aware of the dispute in recent weeks because of warnings being scrolled across their screens.
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