A woman has been arrested and charged over a deadly house fire in Melbourne's north-west last year that killed two children and hospitalised a third.

The 26-year-old woman was arrested in Tarneit on Tuesday and charged with two counts of negligent manslaughter and one count of negligently cause serious injury.

It follows a year-long investigation into the deadly blaze by detectives from the Arson and Explosives Squad.

Emergency services were called to a burning property in Sydenham just after 9:30pm on September 8 last year.

The three children were found unconscious and hospitalised with life-threatening injuries, with two girls dying in hospital three days later.

In the aftermath of the fire, police said detectives believed the blaze began in the rear of the property, while the three

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