Extracellular vesicles (EV) could provide a way of editing genes that does not involve viral vectors, with preclinical studies showing positive results for genetic hearing loss.

Researchers developed a microfluidic electrotransfection platform called μDES to efficiently load EVs with gene editing ribonucleoprotein (RNP).

The microfluidic droplet-based EV electroporation system with continuous flow–based droplet generation and low-voltage electroporation offers high-throughput and scalability while minimizing impact on EV integrity and RNP function.

Xiaoshu Pan, PhD, from the University of Florida, and colleagues report that the μDES had ten times the loading capacity and more than 1000 times the processing throughput for loading RNP complexes into EVs compared with conventional transfec

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